|  Sample Origin:  | 
					Germany | 
				
					|  Source:  | 
					Human HUS | 
				
					|  Depositor:  | 
					S.Brynestad/Y.Wasteson, NVH(FMN/NMH), Oslo, Norway | 	
					|  Passport:  |  H.Schmidt, Würzburg, Germany in 2001  | 
					|  Deposit Date:  | 
					2001-02-05 | 
				
					|   Variant:  | O157:H7 (depositor) | 
					|  Other Collections:  | Schmidt 3538/96(Dstx2::cml); | 
							|   Restriction:  | 
							German hazard group 2, a virulence factor has been deleted in a wildtype strain | 
						
							|  Conditions Remarks:  | 
							Blood agar or LB agar with chloramphenicol [13910] | 
						
							|   Metabolism:  | 
							stx2 gene replaced with cat gene | 
						
							|   Test:  | 
							Recommended O157 | 
						
					|  Remarks:  | Considered a safe and convenient test strain for analysis of O157:H7 verotoxin (or shiga toxin) | 
						|  Literature Reference:  | 
						Appl Environ Microbiol 65:3855-3861,1999 |